Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension Cells

1.9K Views

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08:17 min

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April 26th, 2024

DOI :

10.3791/66532-v

April 26th, 2024

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Kimberly L. Pietersz¹, Paul J.H. Nijhuis¹, Matthijs H.M. Klunder¹, Joëlle van den Herik¹, Barbara Hobo¹, Fred de Winter¹, Joost Verhaagen¹^,²

¹Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences (KNAW), ²Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam

Transcript

Summary

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Adeno associated Vectors

HEK293 Suspension Cells

Viral Vectors

Central Nervous System Repair

Therapeutic Gene Delivery

AAV Protocol

High Yield Production

Gene Therapy

Neurodegenerative Diseases

AAV PHP eB

Transduction Efficiency

Triple Transfection Method

Suspension based Cell Culture

Iodixanol Gradient Ultracentrifugation

Chapters in this video

0:00

Introduction

1:20

HEK293 Suspension Cell Cultivation and Viral Vector Harvesting for Virus Production

4:42

Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno‐Associated Virus Production

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