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Depletion and Reconstitution of Macrophages in Mice

DOI :

10.3791/4105-v

August 1st, 2012

August 1st, 2012

38,942 Views

1Department of Graduate Studies, University of British Columbia , 2Department of Molecular Biology, Vrije Universiteit Amsterdam, 3Department of Pediatrics, University of British Columbia

Macrophages play a central role in homeostasis and pathology in many tissues. The protocol presented here describes methods for depleting macrophages in vivo, deriving polarized macrophages from bone marrow aspirates, and adoptively transferring macrophages into mice. These techniques allow determination of the role that polarized macrophages play in health and disease.

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Depletion

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