Results: Representative Gram-Negative Bacterial Sepsis Imaging in Murine Neonates
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Conclusion
副本
Our protocol allows bacterial dissemination and burden tracking in an experimental neonatal sepsis model in real time, and the ability to correlate other markers of disease with the pathogen burden. This protocol allows us to longitudinally analyz
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Infection of neonatal mice with bioluminescent E. coli O1:K1:H7 results in a septic infection with significant pulmonary inflammation and lung pathology. Here, we describe procedures to model and further study neonatal sepsis using longitudinal intravital imaging in parallel with enumeration of systemic bacterial burdens, inflammatory profiling, and lung histopathology.
