Cell Culture and Expansion of Nasal Epithelial Cells
4:23
Growth and Differentiation of Organoids in Slides and Culture Inserts
6:06
Isolation and Fixation of Organoids
7:36
Preparation and Isolation of Organoids for Histological Sectioning
8:57
Baseline Lumen Measurements
9:56
Results: Live and Fixed Imaging of the Airway Epithelial Organoids Derived from Nasal Epithelial Cells and Analysis Methods
12:21
Conclusion
副本
Individualized therapy for cystic fibrosis patients can be achieved by an in vitro disease model to understand the CFTR activity at baseline. Human nasal epithelial organoids, or HNE organoids, produce lumens of varying sizes that correlate with t
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A detailed protocol is presented here to describe an in vitro organoid model from human nasal epithelial cells. The protocol has options for measurements requiring standard laboratory equipment, with additional possibilities for specialized equipment and software.
