Kidney Fixation, Decolorization, and De-Lipidation
1:45
Whole-Mount Immunofluorescent Staining and Refractive Index (RI) Matching
3:03
Results: Whole-Mount Immunofluorescent Staining for Three-Dimensional (3D) Kidney Imaging
4:00
Conclusion
副本
This protocol allows a visualization of three-dimensional structures in full kidney, which can lead to innovative discoveries in kidney research by providing a macroscopic perspective. Whole-mount organ staining is challenging because of the diffi
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The present protocol describes a tissue clearing method and whole-mount immunofluorescent staining for three-dimensional (3D) kidney imaging. This technique can offer macroscopic perspectives in kidney pathology, leading to new biological discoveries.