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Design und die Verwendung Multiplexed Chemostat Arrays

23.1K Views

19:40 min

February 23rd, 2013

DOI :

10.3791/50262-v

February 23rd, 2013


Weitere Videos entdecken

Genetik

Kapitel in diesem Video

0:01

Title

1:18

How the Ministats Work

2:03

Anatomy of the Array

3:11

How to Clean and Assemble a Ministat Array

3:40

Marking Culture Tubes at the Desired Working Culture Volume

4:11

Making the Cork Assembly

5:48

Setting Up the Gas Washing Bottle to Hydrate Air and Prevent Evaporation

6:39

Use of a 4-Port Manifold to Divide Air Flow Between Multiple Chambers

7:35

Setting the Peristaltic Pump and Heating Block

8:24

Air Tubing Assembly

9:08

Media Tubing Assembly

11:01

Part Assembly and Autoclaving

11:34

Connecting all Tubes to the Culture Chamber

12:33

Preparing the Carboy and Autoclaving

12:55

Running Experiments

15:13

Time Zero Sampling

16:08

Daily Measurements

16:52

Taking Down an Experiment and Cleaning Up

1:12

Design and Use of Multiplexed Chemostat Arrays

8:50

Effluent Tube Assembly

10:21

Preparing Effluent Sampling Containers

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