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JoVE Journal

Neuroscience

This content is Open Access.

Stab Wound Injury of the Zebrafish Adult Telencephalon: A Method to Investigate Vertebrate Brain Neurogenesis and Regeneration

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09:16 min

August 4th, 2014

August 4th, 2014

15,192 Views

0:05

Title

1:32

Generating a Stab Wound in the Telencephalon

2:21

Analyzing the Effect of Telencephalic Injury

4:36

Trimming the Agarose Block and Sectioning the Brain

6:36

Immunohistochemistry on Vibratome Sections

7:28

Telencephalon Stab Wound Results

8:49

Conclusion

To shed light on the cellular and molecular mechanisms of zebrafish adult neurogenesis and regeneration, we developed a protocol for invasive surgery causing mechanical injuries in the zebrafish adult telencephalon and subsequent monitoring of changes in the stabbed hemisphere by immunohistochemistry or in situ hybridization.

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