Construct the pET-SUMO-EGFP Plasmid and Induce EGFP-expression
4:28
Mouse Peritoneal Macrophage Isolation and Primary Culture
6:57
Macrophage Phagocytosis Assay using Flourescence Microscopy
8:28
Macrophage Phagocytosis Assay using Flow Cytometer
11:05
Results
11:45
Conclusion
Transkript
This protocol demonstrated a simple and producible method to assess phagocytosis ability of macrophages using EGFP-expressing Escherichia coli. Hello, my name is Jun-Yu from The Second Hospital of Dalian Medical University. Today we are going to s
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Here, we present a protocol to assess mouse peritoneal macrophage phagocytosis using enhanced green fluorescence protein-expressing Escherichia coli.