Disintegration and Cell Suspension of Tumor Spheroids
5:02
Cancer-Associated Fibroblast (CAF) Isolation Through Microbeads
6:19
Flow Cytometry-Based Analysis of ACTA2 Expression in Isolated CAFs
7:28
Results: Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts
9:30
Conclusion
Transkript
This protocol highlights the importance of the tumor microenvironment in activating the cancer-associated fibroblasts or CAF. It could be excellent in vitro model to study phenotypic characteristics. The major constraints in CAF biology lies in th
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Multicellular 3D tumor spheroids were prepared with lung adenocarcinoma cells, fibroblasts, and monocytes, followed by the isolation of cancer-associated fibroblasts (CAFs) from these spheroids. Isolated CAFs were compared with normal fibroblasts to assess mitochondrial health by studying the mitochondrial transmembrane potential, reactive oxygen species, and enzymatic activities.