Evaluating Suitability of Embryos for Cryopreservation
3:58
Washing Embryos to Reduce Likelihood of Disease Transmission
5:20
Dehydrating Embryos and Loading Embryos into a 0.25 ml Plastic Straw
7:11
Freezing Embryos
8:53
Thawing Cryopreserved Embryos
10:26
Results of Transferring Frozen-thawed Embryos to Female Recipients
10:45
Conclusion
Preimplantation embryos may be cryopreserved after placement into a hypertonic cryoprotective solution to cause cellular dehydration. After equilibration, ice crystal formation is induced in the solution surrounding the embryo. Further dehydration occurs as the embryo is slowly cooled to subzero temperatures before plunging into liquid nitrogen for storage.