Preparation of Polysome Lysate and Sucrose Gradient Fractionation
3:40
Atomic Force Microscopy Sample Preparation
5:24
AFM Imaging
6:40
Results: AFM Reveals Ribosome/Polysome Details
7:39
Conclusion
Transcripción
The overall goal of this protocol is to provide a detailed pipeline for the accurate purification and deposition of brain polyribosomes on mica and to obtain thousands of images at the nanoscale resolution without the need for heavy post-processin
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While ribosome structure has been extensively characterized, the organization of polysomes is still understudied. To overcome this lack of knowledge, we present here a detailed preparation protocol for accurate imaging of mammalian polysomes by atomic force microscopy (AFM) in air and liquid.