Expression of Wild-type BM3 (Bacterial Cytochrome P450 Enzyme)
2:10
Purification of BM3
5:35
Extraction of EDPs (Epoxydocosapentaenoic Acids)
7:23
Esterification of EDPs, Separation of 16(S), 17(R)- and 19(S),20(R)-EDP, and Saponification of Esters
11:17
Results: Chromatogram and 1H-NMR Spectra
12:13
Conclusion
Transcripción
So our protocol allows us to prepare enantiopure epoxy fatty acids, and this method might help advance research on the mechanisms by which these metabolites act in the body. Historically, pure epoxy fatty acids are very difficult to obtain. So our
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We present a method useful for large-scale enzymatic synthesis and purification of specific enantiomers and regioisomers of epoxides of arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) with the use of a bacterial cytochrome P450 enzyme (BM3).