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JoVE Journal

Cancer Research

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Measuring Real-time Drug Response in Organotypic Tumor Tissue Slices

10.9K Views

08:27 min

May 2nd, 2020

May 2nd, 2020

10,948 Views

0:04

Introduction

1:12

Tumor Tissue Slice Preparation

4:13

Viability Measurement

5:07

Drug Effect Evaluation

5:58

Results: Representative Tissue Slice Viability Measurement and Drug Response

7:33

Conclusion

Transcribir

Our protocol is compatible with other molecular analysis methods and allows the assessment of how drugs affect the viability of in-tact tissue slices and the performance of medium throughput screening. The main advantage of this technique is that

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We introduce a protocol for measuring real-time drug response in organotypic tumor tissue slices. The experimental strategy outlined here provides a platform to carry out medium-high throughput drug screens on tissue slices derived from clinical or mouse tumors in ex vivo conditions.

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