Results: Effects of MMC on Cell Protein and Gene Expression
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Conclusion
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We created an individual collagen-rich model to mimic the environment that cells experience in vivo using the macromolecular crowding technique. Skin is a crowded environment, unlike the liquid environment that we already use to grow cells in vitr
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This protocol describes the use of macromolecular crowding to create an in vitro human hypertrophic scar tissue model that resembles in vivo conditions. When cultivated in a crowded macromolecular environment, human skin fibroblasts exhibit phenotypes, biochemistry, physiology, and functional characteristics resembling scar tissue.