Media Collection, Imaging, and Downstream Processing
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Results: Analysis of H & E Staining, Number of Follicles at Different Stages, and Collagen Staining of the Ovarian Cortex
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Conclusion
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This protocol allows for an in vitro in depth examination of the ovarian micro environment. Enabling users to determine follicle growth and development, as well as steroidogenesis and abundance of immune molecules. One advantage of this technique
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In vitro culture of bovine ovarian cortex and the effect of nutritional Stair-step diet on ovarian microenvironment is presented. Ovarian cortex pieces were cultured for seven days and steroids, cytokines, and follicle stages were evaluated. The Stair-Step diet treatment had increased steroidogenesis resulting in follicle progression in culture.