Results: Visualizing Mitophagy Triggered by FCCP Stimulation
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Conclusion
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This method helps to observe mitophagy in living cells using a cell permeant green-fluorescent mitochondria dye and a red-fluorescent lysosome dye. Mitophagy plays an important role in maintaining mitochondria homeostasis and various aspects of ce
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Mitophagy is the primary mechanism of mitochondrial quality control. However, the evaluation of mitophagy in vivo is hindered by the lack of reliable quantitative assays. Presented here is a protocol for the observation of mitophagy in living cells using a cell-permeant green-fluorescent mitochondria dye and a red-fluorescent lysosome dye.