Preparing the Kernels for Photography and Analysis
6:07
Results: Analysis of the Transgenic Maize
8:23
Conclusion
Transcription
Here we present a simple kernel screening assay to analyze Aspergillus flavus growth and Aflatoxin production in maize kernels expressing an antifungal protein. Using a green fluorescent protein expressing Aspergillus flavus strain, we monitor the
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Here we present a protocol to analyze Aspergillus flavus growth and aflatoxin production in maize kernels expressing an antifungal protein. Using a GFP-expressing A. flavus strain we monitored the infection and spread of the fungus in mature kernels in real time. The assay is rapid, reliable, and reproducible.