Cloning Parts into the Entry Vector (pYTK001) to Create Part
2:05
Assembling Part Plasmids into Cassette Plasmids
4:50
Assembling Cassette Plasmids into Multi-Gene Plasmids
5:55
Results: Multi-gene Plasmid Cloning in E. coli and Yeast
7:54
Conclusion
Transcription
This is a detailed protocol for assembling multi-gene plasmids using the Yeast MoClo kit. This method enables the convenient cloning of a variety of multi-gene classmates. It is ideal for generating a big library of related parts.
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The goal of this protocol is to provide a detailed, step-by-step guide for assembling multi-gene constructs using the modular cloning system based on Golden Gate cloning. It also gives recommendations on critical steps to ensure optimal assembly based on our experiences.