15.8K Views
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13:51 min
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July 11th, 2013
DOI :
July 11th, 2013
•0:05
Title
2:13
Tandem Recombineering (TR) for Creating Multigene Expression Constructs
4:57
Composite Multigene Baculovirus Generation, Amplification and Storage
5:02
Baculovirus Generation
6:42
Amplification
8:14
Storage
8:55
Protein Production and Downstream Processing
8:57
Infecting Larger Cultures and Monitoring YFP
10:20
Cell Lysis and Fractionation
10:45
Protein Purification
11:07
Future Outlook
11:33
Results: Production of Eukaryotic Protein Complexes by MultiBac
12:18
Conclusion
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