Plating, Pulsing, Fixation, Permeabilization, and Blocking of Cells
3:59
Immunostaining with the IdU Antibody
5:15
Results: IdU-Induced Replication Stress
5:41
Conclusion
Transcription
Our protocol presents a highly efficient and easily reproducible method to quantify single stranded DNA, which is a marker of replication stress in a variety of cell lines. Additionally, its simplicity allows for high throughput applications and s
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Here, we describe an immunofluorescence-based method to quantify the levels of single-stranded DNA in cells. This efficient and reproducible method can be utilized to examine replication stress, a common feature in several ovarian cancers. Additionally, this assay is compatible with an automated analysis pipeline, which further increases its efficiency.