In bacteria, the study of the dynamics of cell division proteins requires a spatial orientation of the cells. For example, in the case of the Z-ring, the main component in bacterial cell division, a vertical orientation is fundamental to record th
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We present a simple and accessible method for filamentous cyanobacterial visualization in the XY plane. A low-melting point agarose matrix was used, allowing the acquisition of images of proteins involved in the division, in a vertical orientation. Therefore, this methodology can be applied to any filamentous organism and different kinds of proteins.