Manual Fabrication of the Mammary Imaging Window Window (MIW)
2:58
Insertion of MW
5:28
Using the Imaging Box
6:39
Photoswitching and Imaging of Dendra2-labeled Cells
8:09
Representative Results: Photoswitching
8:39
Conclusion
Intravital photoswitching and tracking of Dendra2-labeled tumor cells through the Mammary Imaging Window is a technique which allows us to image the metastatic behavior of tumor cells in chosen tumor microenvironments over a timescale of days.