Preparing Microinjection Needles, Oil Injector Apparatus, and Embryos
2:28
Draining and Collecting the Embryonic Cerebrospinal Fluid (eCSF)
4:24
Representative eCSF Drainage Results
5:55
Conclusion
We present a method to collect cerebrospinal fluid (CSF) and to create a system which lacks CSF within the embryonic zebrafish brain ventricular system. This allows for further examination of CSF composition and its requirement during embryonic brain development.