Isolating the human muscle progenitor cells that regulate skeletal muscle regeneration allows us to investigate processes that underlie the regenerative process while retaining donor variability. This technique allows large yields of human muscle
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We present techniques for isolating, culturing, characterizing, and differentiating human primary muscle progenitor cells (hMPCs) obtained from skeletal muscle biopsy tissue.hMPCs obtained and characterized through these methods can be used to subsequently address research questions related to human myogenesis and skeletal muscle regeneration.