The overall goal of this immunofluorescent staining protocol is to monitor the autophagic process in muscle satellite cells during skeletal muscle regeneration. This method can help answer key questions in the tissue regeneration field about under
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Active autophagy is associated with productive muscle regeneration, which is essential for Muscle Stem Cell (MuSC) activation. Here, we provide a protocol for the in situ detection of LC3, an autophagy marker in MyoD-positive MuSCs of muscle tissue sections from control and injured mice.