Post-grafting Management, Cold Treatment, and Sampling
3:45
Library Preparation and High-throughput Sequencing
5:20
Results: miRNA Up- and Down-regulated in Cold Treated Homografts and Heterografts
6:17
Conclusion
필기록
This method can help answer key questions in miRNA response to ability to stress in plant grafts such as how miRNA are regulated under cold stress in watermelon bottle gourd grafts. The main advantage of this technique is that it is highly efficie
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Here we present a detailed protocol for efficiently making homo- and heterografts between watermelon and bottle gourd, in addition to methods of tissue sampling, data generation, and data analysis, for the investigation of cold-responsive microRNAs.