Results: Apparent Kinetics of Tight Junction Barrier Formation in hCMEC/D3 cells
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Conclusion
필기록
This protocol is a robust technique to determine the functional integrity of intercellular tight junctions in a cell model of the human blood-brain barrier. This is a simple and fairly inexpensive protocol that allows calculating the apparent perm
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We present a fluorescence assay to demonstrate that Lucifer Yellow (LY) is a robust marker to determine the apparent paracellular permeability of hCMEC/D3 cell monolayers, an in vitro model of the human blood-brain barrier. We used this assay to determine the kinetics of a confluent monolayer formation in cultured hCMEC/D3 cells.