Preparation of Each Bacterial Strain for Incubation
1:21
Coincubation of Strains
2:54
Serial Dilution for Starting Inoculum
4:37
Taking T Final Measurements
5:36
Results: Quantifying Competitive Interactions Between the Strains
7:26
Conclusion
필기록
Our protocol provides a simple yet robust approach to identify and characterize competitive mechanisms between different strains of Vibrio fischeri bacteria. This method uses stable plasmids that encode different antibiotic resistance genes and fl
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Bacteria encode diverse mechanisms for engaging in interbacterial competition. Here, we present a culture-based protocol for characterizing competitive interactions between bacterial isolates and how they impact the spatial structure of a mixed population.