May 19th, 2020
•The goal of this protocol is to analyze cell division in intact tissue by live and fixed cell microscopy using Drosophila meiotic spermatocytes. The protocol demonstrates how to isolate whole, intact testes from Drosophila larvae and early pupae, and how to process and mount them for microscopy.
Tags
Related Videos
Live-imaging of the Drosophila Pupal Eye
Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
Live Imaging of Mouse Secondary Palate Fusion
Methods for Imaging Intracellular pH of the Follicle Stem Cell Lineage in Live Drosophila Ovarian Tissue
Dissection and Staining of Drosophila Pupal Ovaries
Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation
Live Imaging and Analysis of Muscle Contractions in Drosophila Embryo
Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages
Copyright © 2024 MyJoVE Corporation. 판권 소유