Protocol: Phase II- Preparation of Alcohol Insoluble Material (Water and Methanol Washes)
3:46
Protocol: Phase III- Treatment of Pellet with TGA and Acid to Precipitate Lignin
6:22
Protocol: Phase IV- Results (Ab280mm Reading)
7:01
Representative Results
8:38
Conclusion
필기록
Lignin is a complex heteropolymer made up of three monolignols. It is the second most abundant polymer on earth next to cellulose. Here, we demonstrate thioglycolic acid method, which is the most reliable method for the estimation of lignin conten
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Here, we present a modified TGA method for estimation of lignin content in herbaceous plant biomass. This method estimates the lignin content by forming specific thioether bonds with lignin and presents an advantage over the Klason method, as it requires a relatively small sample for lignin content estimation.