Results: Protein Expression from the Rolling Circle Amplification (RCA) Templates Using Cell-Free Systems
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Conclusion
필기록
This protocol is significant because it details how to quickly generate large amounts of DNA template for use in cell-free reactions from a small gene fragment received from a synthesis facility. Rolling circle amplification can generate large amo
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The study describes a protocol for creating large (µg-mg) quantities of DNA for protein screening campaigns from synthetic gene fragments without cloning or using living cells. The minimal template is enzymatically digested and circularized and then amplified using isothermal rolling circle amplification. Cell-free expression reactions could be performed with the unpurified product.