Seeding Cells into ECM Basement Material-Coated Plates
2:41
Image Acquisition of Spheroids
3:07
Image Acquisition of Spheroids
4:35
Results: Observation and Imaging of Spheroids
6:07
Conclusion
필기록
This protocol provides a method to produce several hundred multicellular tumorous spheroids in each well of a multi well plate suitable for automated microscopy. Spheroid produced can be categorized into different size classes, and subcellular inf
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요약
This protocol details a method for the production of three different types of spheroids in a manner that makes them suitable for large-scale high-content screening and analysis. In addition, examples are presented showing how they can be analyzed at spheroid and individual cell levels.