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A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications

2.9K Views

06:40 min

December 28th, 2021

December 28th, 2021

2,943 Views

0:05

Introduction

0:35

Coating 96-Well Plate with ECM Basement Material

1:07

Subculturing and Cell Counting

1:52

Seeding Cells into ECM Basement Material-Coated Plates

2:41

Image Acquisition of Spheroids

3:07

Image Acquisition of Spheroids

4:35

Results: Observation and Imaging of Spheroids

6:07

Conclusion

필기록

This protocol provides a method to produce several hundred multicellular tumorous spheroids in each well of a multi well plate suitable for automated microscopy. Spheroid produced can be categorized into different size classes, and subcellular inf

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This protocol details a method for the production of three different types of spheroids in a manner that makes them suitable for large-scale high-content screening and analysis. In addition, examples are presented showing how they can be analyzed at spheroid and individual cell levels.

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