Fabrication of the Janus Base Nano-Matrix (JBNm), Composed of Janus Base Nanotube (JBNt)/Matn1/Transforming Growth Factor Beta-1 (TGF-β1)
1:41
Observation of Specimens with Ultraviolet-Visible (UV-Vis) Spectroscopy
3:12
Absorption Spectra Measurement of Fluorescent-Labeled Proteins
5:08
Results: Layer-By-Layer Assembly of Janus Base Nano-Matrix Structure Scaffold for a Cartilage Tissue Construct
8:01
Conclusion
필기록
This protocol shows how we assembled and characterized the layer by layer JBNm at a molecular level. JBNm encapsulates TGF-B1, and prevents its release in the surrounding tissue, thus promoting localized chondrogenesis. The formation of the JBNm i
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This protocol describes the assembly of a layer-by-layer Janus base nano-matrix (JBNm) scaffold by adding Janus base nanotubes (JBNts), matrilin-3, and Transforming Growth Factor Beta-1 (TGF-β1) sequentially. The JBNm was fabricated and characterized; additionally, it displayed excellent bioactivity, encouraging cell functions such as adhesion, proliferation, and differentiation.