Sampling and TBO Staining of Arabidopsis Cotyledons
1:58
Peptide Treatment and Imaging
3:24
Results: The Effect of the Incubation Time with TBO on the Genotypes, TBO-Stained Epidermis Images, and Confocal Images of Cotyledon Epidermis with or without Peptide Treatment
4:27
Conclusion
필기록
The two phenotypic analysis methods presented here offers high-quality images that are sufficient for conducting quantitative phenotypic analysis and for demonstrating phenotypic responses of peptide treatment with epidermal details. Consequently,
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This paper describes two phenotyping methods without the use of epidermal peels to characterize the genes controlling stomatal development. The first method demonstrates how to analyze a stomatal phenotype using a toluidine blue O-stained plant epidermis. The second method describes how to identify stomatal ligands and monitor their biological activities.