A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

Podsumowanie

This article describes a method for studying cellular metabolism in complex communities of multiple cell types, using a combination of stable isotope tracing, cell sorting to isolate specific cell types, and mass spectrometry.

Streszczenie

Mammalian cell types exhibit specialized metabolism, and there is ample evidence that various co-existing cell types engage in metabolic cooperation. Moreover, even cultures of a single cell type may contain cells in distinct metabolic states, such as resting or cycling cells. Methods for measuring metabolic activities of such subpopulations are valuable tools for understanding cellular metabolism. Complex cell populations are most commonly separated using a cell sorter, and subpopulations isolated by this method can be analyzed by metabolomics methods. However, a problem with this approach is that the cell sorting procedure subjects cells to stresses that may distort their metabolism.

To overcome these issues, we reasoned that the mass isotopomer distributions (MIDs) of metabolites from cells cultured with stable isotope-labeled nutrients are likely to be more stable than absolute metabolite concentrations, because MIDs are formed over longer time scales and should be less affected by short-term exposure to cell sorting conditions. Here, we describe a method based on this principle, combining cell sorting with liquid chromatography-high resolution mass spectrometry (LC-HRMS). The procedure involves analyzing three types of samples: (1) metabolite extracts obtained directly from the complex population; (2) extracts of "mock sorted" cells passed through the cell sorter instrument without gating any specific population; and (3) extracts of the actual sorted populations. The mock sorted cells are compared against direct extraction to verify that MIDs are indeed not altered by the cell sorting procedure itself, prior to analyzing the actual sorted populations. We show example results from HeLa cells sorted according to cell cycle phase, revealing changes in nucleotide metabolism.

Wprowadzenie

Higher organisms contain complex communities of distinct cell types that collaborate to bring about more complex functions. For example, tumors contain not only cancerous cells, but also fibroblasts, cells that constitute blood vessels, and often immune cell infiltrates¹; blood contains a complex mixture of dozens of immune cell subtypes²; and even cultured cell lines may consist of multiple subpopulations, such as the luminal and basal subtypes of breast cancer cells³. Moreover, distinct cell types that coexist can exhibit metabolic "collaboration". For example, in the brain, astrocytes are thought to convert glucose to lactate, which is then "fed" to neurons that oxidize this substrate⁴; T lymphocytes are in some contexts dependent on adjacent dendritic cells as a source of cysteine⁵; and cancer cells may collaborate with associated fibroblasts in tumors⁶. To understand the metabolic behavior of such systems, it is essential to separate and measure the metabolic activities of the various cell types present.

By far the most widely used method for separating cell types is fluorescence-activated cell sorting. This method is broadly applicable, provided that the cell type or state of interest can be "labeled" using fluorescent antibodies, expression of engineered fluorescent proteins, or other dyes. One option is to initially separate cells types through a cell sorter, re-culture the individual cell types obtained, and then perform metabolism studies of these cultures⁷. However, this is only feasible if the cell type or phenotype is stable in culture conditions, and cannot capture transient behavior such as cell cycle states, nor the metabolic cooperation in co-cultures. For such cases, metabolism must be measured directly on sorted cells. This is challenging since the cell sorting procedure subjects cells to stresses that may distort their metabolism⁸, and we are aware of only a few studies taking this approach^9,10. In particular, we have found that major metabolites such as amino acids may leak from cells kept in cell sorting buffer, so that measurements of absolute metabolite abundance are no longer reliable¹¹ (although relative comparison between sorted fractions may still be valuable).

To circumvent these issues, we label cells with stable isotopes prior to sorting, and focus on the MIDs in cellular metabolites, rather than metabolite abundances. Since MIDs are formed over longer time scales, they should be less affected by short-term exposure to sorting conditions. We quantify MIDs using full-scan high-resolution mass spectrometry, which is sensitive enough to provide data on hundreds of metabolites starting from around 500,000 sorted cells, requiring about 30-60 min of cell sorting time. A comparison between a "mock sorted" control (cells passed through the cell sorter instrument without gating any specific population) and metabolite extraction directly from the culture dish is made to ensure that the observed MIDs are representative of those in the original culture. Depending on the choice of stable isotope tracers, various metabolic pathways can be studied with this method.

Protokół

1. Metabolite Extraction

1. Extraction from dish
   1. Culture cells in a 6-well plate in triplicates in stable isotope labeled culture media + dialyzed supplements (serum or other growth supplements) until cells become 75% confluent.
      NOTE: Here culture HeLa cells for 48 h in RPMI containing 40% U-¹³C-Glucose and 70% U-¹³C, ¹⁵N2-Glutamine and 5% dialyzed FBS (Fetal Bovine Serum). Dialyzed FBS is used to get rid of the small molecular weight metabolites which might contaminate the labeled media. Culturing cells in dialyzed supplement prior to the real experiment is recommended to ensure cells are growing normally in the medium. Supplements are dialyzed in 0.15 M NaCl solution overnight using snake skin dialysis tubing.
   2. At the day of extraction discard culture media, rinse wells twice with 500 µL cold HBSS and then discard it.
      NOTE: Here use HBSS containing 40% U-¹³C-Glucose since it is also in the culture media.
   3. Add 600 µL 100% methanol pre-cooled on dry ice.
   4. Transfer the dish to dry ice and remove cell material with a cell scraper.
   5. Carefully pipette the cell extracts to a microcentrifuge tube and store at -80 °C until mass spectrometry analysis.
2. Extraction of mock sorted cells
   1. Culture cells in a 100 mm dish in triplicates in stable isotope labeled culture media + dialyzed supplements.
      NOTE: Here culture HeLa cells for 48 h in 100 mm dish because a high number of cells (~4 x 10⁶) are needed to obtain 500,000 sorted cells. This number of HeLa cells extract was required to obtain good measurement of metabolites. The culture media used was RPMI containing 40% U-¹³C-Glucose and 70% U-¹³C, ¹⁵N2-Glutamine and 5% dialyzed FBS.
   2. At the day of extraction, discard culture media, rinse wells with 1.5 mL warm HBSS and then discard it.
   3. Detach cells by adding 1.5 mL trypsin/EDTA for 4 min at 37 °C. Perform the following steps in 4 °C or in ice.
   4. Deactivate trypsin by adding 3 mL ice cold HBSS (Hank's Balanced Salt Solution) + dialyzed supplement.
   5. Collect cells in a 15 mL tube and centrifuge at 750 x g for 3 min.
   6. Resuspend the pellet in HBSS + dialyzed supplement + 1 mM EDTA at a concentration 1-2 x 10⁶ cells/mL, pass through 40 µm cell strainers to obtain single cells, and transfer to a 5 mL tube.
   7. Sort cells through cell sorter gating only for singlets, and centrifuge sorted cells at 750 x g for 3 min at 4 °C.
      NOTE: Here sort HeLa cells at a rate 1,000 events/s, with instrument pressure 27 psi, and using a 100 µm nozzle. HeLa cells are big so they should be sorted at a slow event rate and large nozzle to get as intact pellet as possible. Keep the cells in cold blocks throughout sorting to decrease the metabolism. A thorough description of sorting procedure is described previously¹².
   8. Discard the supernatant and resuspend the pellet in 50 µL ice cold dH₂O to obtain a homogenous pellet before adding methanol. Addition of cold methanol directly to pellet forms a solid pellet which is hard to resuspend.
   9. Extract metabolites by adding 540 µL methanol kept in dry ice and keep extracts at -80 °C until liquid chromatography – high resolution mass spectrometry (LC-HRMS) analysis.
3. Extraction of cell cycle sorted cells
   1. Culture cells in a 100 mm dish in triplicates in culture media + dialyzed supplements.
      NOTE: Here culture HeLa cells containing Geminin Fucci Green (mAG1-hGem) probe¹³ which allows for sorting of G1 (negative) and SG2M (positive) cells. Cells can be cultured in stable isotope tracing media for as long as required. Here we have cultured them for 46 h in unlabeled media, then we have switched to RPMI containing 40% U-¹³C-Glucose and 70% U-¹³C, ¹⁵N2-Glutamine 2 h before starting sorting. This was done in order to be able to study the cell cycle phases which requires a short pulse labeling.
   2. At the day of extraction, discard culture media, rinse wells with 1.5 mL warm HBSS and then discard it.
   3. Detach cells by adding 1.5 mL trypsin/EDTA for 4 min at 37 °C. Perform the following steps in 4 °C or in ice.
   4. Deactivate trypsin by adding 3 mL ice cold HBSS + dialyzed supplement.
   5. Collect cells in a 15 mL tube and centrifuge at 750 x g for 3 min.
   6. Resuspend the pellet in HBSS + dialyzed supplement + 1 mM EDTA at a concentration 1-2 x 10⁶ cells/mL, pass through 40 µm cell strainers to obtain single cells, and transfer to a 5 mL tube.
   7. Sort cells through the cell sorter gating out debris and doublets, then gating for the cell marker of interest, and centrifuge sorted cells at 750 x g for 3 min at 4 °C.
      NOTE: Here sort HeLa cells at a rate 1,000 events/s, with instrument pressure 27 psi, and using a 100 µm nozzle. Keep the cells in cold blocks throughout sorting to decrease the metabolism rate.
   8. Discard the supernatant and resuspend the pellet in 50 µL ice cold dH₂O to obtain a homogenous pellet before adding methanol. Addition of cold methanol directly to pellet forms a solid pellet which is hard to resuspend.
   9. Extract the metabolites by adding 540 µL methanol kept in dry ice and keep extracts at -80 °C until LC-HRMS analysis.

2. Mass Spectrometry Analysis

Note: Here we describe the protocol for analyzing cell extracts on a LC-HRMS system. Any metabolomics methods for analysis of cell extracts can be used. Full scan analysis might be useful for detecting a wide range of metabolites.

1. Calibrate the instrument using a mass spectrometry reference calibration mix.
2. Thaw cell extracts on ice for 30 min and vortex for 15 s.
3. Transfer 100 µL of the cell extract to a spin filter and centrifuge for 10 min at 13,000 x g at 4 °C.
4. Inject 12.5 µL of the filtrate onto the LC-HRMS system.
5. Separate metabolites using a Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) column (150 mm x 4.6 mm, 5 µm particle size) fitted with a ZIC-HILIC guard column (20 mm × 2.1 mm) using a gradient elution of 0.1% formic acid in water (solvent A) and acetonitrile (solvent B). Start the gradient elution at 20% of solvent A and increase up to 80% in 17 min. Maintain this percentage during 4 min with a flow of 400 µL min^-1 and column temperature and sample tray at 23 °C and 4 °C, respectively.
6. Use an instrument coupled to the chromatographic separation for metabolites detection, a heated electrospray (H-ESI II) in both positive and negative modes as ionization source, and a full scan acquisition mode at a mass resolving power of 70,000 Full Width Half Maximum (FWHM) (m/z 200).
7. Use nitrogen (purity >99.995%) for the sheath gas and auxiliary gas at a flow rate of 45 and 10 a.u. (arbitrary units) and set vaporizer temperature at 350 °C and the electrospray voltage at 4 kV in positive mode and -3.5 kV in negative mode.

3. Data Analysis

1. Select a number of metabolites for which standards are available and which show good quality peaks in the samples. Good quality peaks have high signal to noise ratio. It is important to verify peak quality and make sure not to include false isotopes. Isotopic peaks which differ in shape and/or retention time are likely false.
2. For isotope-labeled samples calculate mass isotopomer (MI) fractions by dividing the peak area of each MI with total peak areas of all MIs.
3. Calculate the labeled carbon/nitrogen fractions, enrichment of ¹³C and ¹⁵N, respectively, as
   
   where n is the total number of carbons (or nitrogens, respectively) in the metabolite, and MIx is the MI fraction of x.
   NOTE: All calculations can be performed using programming languages.

Wyniki

As an example, here we describe an experiment investigating the metabolism of HeLa cells sorted according to cell cycle phase. To label a wide range of central metabolites on both carbons and nitrogens, we cultured cells for 48 h using U-¹³C-glucose and U-¹³C, ¹⁵N-glutamine as tracers. To obtain rich MIDs for the validation experiment, we here chose a mixture of 40% U-¹³C-Glucose and 70% U-¹³C,¹⁵N2-Glutamine, as intermediat...

Dyskusje

Our method is based on the principle that MIDs in cellular metabolites reflect the "history" of metabolic activities of a cell. This makes it possible to investigate metabolic activities in subpopulation of cells, as they occurred in the complex community of cells, prior to the cell sorting procedure. In contrast, peak areas of metabolites differ markedly between extracts of sorted cells and direct extraction from the culture dish¹¹. In part this is because the different chemical compositi...

Materiały

Name	Company	Catalog Number	Comments
HBSS	Sigma	H6648
INFLUX (inFlux v7 Sorter)	BD Biosciences
U-13C-Glucose	Cambridge isotopes	40762-22-9 / GLC-018
U-13C,15N2-Glutamine	Cambridge isotopes	CNLM-1275-H-0.1
Methanol (JT Baker), HPLC grade	VWR	BAKR8402.2500
Ultrafree - MC - VV centrifugal Filters. Durapore PVDF 0.1 µm	Millipore	UFC30VV00
Ultimate 3,000 UHPLC	Thermo Fisher scientific
Q-Exactive Orbitrap Mass spectrometer	Thermo Fisher scientific
Merk-Sequant ZIC HILIC column (150 mm x 4.6 mm, 5 µm)	Merck KGaA	1.50444.0001
Merk-Sequant ZIC HILIC guard column (20 mm x 2.1 mm)	Merck KGaA
Acetonitrile Optima LC-MS, amber glass	Fisher Scientific	A955-212
Milli-Q water	Millipore		Produced with a Milli-Q Gradient system
Myrsyra 99.5% Optima (Formic acid)	Fisher Scientific	11423423
X100 Screw Vial 1.5 ml, 8-425 32x11.6 mm, amber, 100 units	Thermo Fisher scientific	10560053
X100 Lock Skruv Vitt PTFE Packing 8-425 (Screw caps)	Thermo Fisher scientific	12458636
ProteoMass LTQ/FT-Hybrid ESI Pos. Mode Cal Mix	Sigma-Aldrich	MSCAL5	Calibration kit
SNAKESKIN 10K MWCO	Thermo Fisher scientific	88245
Mathematica v.10	Wolfram Research