Lipid-containing Oil Solution and Small Vesicle (SV) Preparation
1:54
Bacterial Cell Pre-culture
2:45
Giant Vesicle (GV) and Water-in-Oil (W/O) Bacterial Cell-containing Droplet Formation
4:11
Handmade Chamber and Supported Bilayer Membrane Preparation
4:54
GV Immobilization and Microscopic Bacterial Growth Observation
6:04
Results: Representative GV Containing Bacteria and Bacterial Growth Evaluation
6:44
Conclusion
副本
This protocol provides a means for culturing bacterial cells at the single-cell level inside giant vesicles. It's easy to develop the giant vesicles, and only a very small volume of sample solution is required to prepare the vesicles. Demonstratin
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We demonstrate single-cell culture of bacteria inside giant vesicles (GVs). GVs containing bacterial cells were prepared by the droplet transfer method and were immobilized on a supported membrane on a glass substrate for direct observation of bacterial growth. This approach may also be adaptable to other cells.
