Detection of Abnormal Prion Protein by Immunohistochemistry

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2.7K Views

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06:38 min

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May 5th, 2023

DOI :

10.3791/64560-v

May 5th, 2023

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Leonor Orge¹^,², Maria de Lurdes Pinto¹, Paula Cristovão², Paula Mendonça², Paulo Carvalho², Carla Lima³, Helena Santos², Anabela Alves¹, Fernanda Seixas¹, Isabel Pires¹, Adelina Gama¹, Maria dos Anjos Pires¹

¹Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Science-AL4AnimalS, University of Trás-os-Montes and Alto Douro (UTAD), ²Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Oeiras, ³Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Vairão

Podsumowanie

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Abnormal Prion Protein

Endogenous Peroxidase Inactivation

Tris buffered Saline

Immunodetection

Rozdziały w tym wideo

0:04

Introduction

0:48

Deparaffinization and Rehydration

1:39

Epitope Retrieval

2:31

Inactivation of Endogenous Peroxidase and Immunodetection

4:59

Results: Epitope Demasking for Proper PrP Immunolabeling and Minimizing Non-Specific Background Staining

6:00

Conclusion

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