Results: The Morphology and Viability of PDL Cellular Spheroids on Chitosan Films
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Conclusion
Transkript
This is a detailed protocol of culturing periodontal ligament cell spheroids by chitosan films. Compared with the conventional culture system, the spheroid culture system produces periodontal ligament cells with increased self-renewal and osteogen
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Here, we present protocols of culturing human periodontal ligament (PDL) cell spheroids by chitosan films. The culture of three-dimensional (3D) cellular spheroids provides an alternative to conventional tissue culture polystyrene (TCPS) culture system.