This method allows for the determination of natural killer cell metabolism. A key parameter in the activation by measuring oxygen consumption and pH changes in the extracellular medium. The advantage of the extracellular flux analyzer is that it i
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
In this paper, we describe a method to measure glycolysis and mitochondrial respiration in primary human Natural Killer (NK) cells isolated from peripheral blood, at rest or following IL15-induced activation. The protocol described could be easily extended to primary human NK cells activated by other cytokines or soluble stimuli.