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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

DOI :

10.3791/2925-v

9:45 min

February 9th, 2012

February 9th, 2012

24,272 Views

1Department of Physics, King's College London, 2Department of Chemistry, Imperial College London , 3PhotoBiotics Ltd

Fluorescence Lifetime Imaging (FLIM) has emerged as a key technique to image the environment and interaction of specific proteins and dyes in living cells. FLIM of fluorescent molecular rotors allows mapping of viscosity in living cells.

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Fluorescence Lifetime Imaging

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