Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

39.1K Views

14:49 min

October 27th, 2011

DOI :

10.3791/3340-v

October 27th, 2011


Explore More Videos

MRNA Seq

Chapters in this video

0:05

Title

2:02

Procedural Flow Chart

3:19

cDNA Synthesis

6:31

End Repair, Paired End Library

7:37

Adding 'A' Bases to the 3' End of the DNA Fragments

8:31

Adapter Ligation

10:32

Size Selection/Gel Purification

11:20

Evaluation of Single-Read and Paired-End Sequencing Libraries

14:18

Conclusion

Related Videos

article

16:05

Large-Scale Screens of Metagenomic Libraries

8.6K Views

article

17:25

RNA Extraction from Neuroprecursor Cells Using the Bio-Rad Total RNA Kit

8.6K Views

article

18:17

Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

30.6K Views

article

10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

15.0K Views

article

08:55

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

18.1K Views

article

21:55

Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation

30.6K Views

article

07:28

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

7.6K Views

article

06:00

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

4.2K Views

article

01:58

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

2.9K Views

article

02:02

Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing

500 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved