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Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

39.1K Views

14:49 min

October 27th, 2011

DOI :

10.3791/3340-v

October 27th, 2011


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MRNA Seq

Chapters in this video

0:05

Title

2:02

Procedural Flow Chart

3:19

cDNA Synthesis

6:31

End Repair, Paired End Library

7:37

Adding 'A' Bases to the 3' End of the DNA Fragments

8:31

Adapter Ligation

10:32

Size Selection/Gel Purification

11:20

Evaluation of Single-Read and Paired-End Sequencing Libraries

14:18

Conclusion

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