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Reverse Genetics Mediated Recovery of Infectious Murine Norovirus

16.5K Views

13:48 min

June 24th, 2012

DOI :

10.3791/4145-v

June 24th, 2012


Transcript

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Reverse Genetics

Chapters in this video

0:05

Title

1:34

Procedure Overview

3:16

RNA Transcription and Capping for the Recovery of Infectious MNV

6:27

MNV Recovery by RNA Electroporation into Raw Cells

8:25

Recovery by Lipofection into BSR-T7 Cells

10:07

Direct Recovery of Infectious MNV from cDNA in Cells Expressing T7 RNA Polymerase

12:20

Representative Results

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