Protoplast Transformation using Polyethylene Glycol
3:53
Analysis of the Protein Import into Chloroplasts
5:10
Results: Fluorescence Microscopy and Immunoblotting to Analyze the Import of Proteins into Chloroplasts
6:07
Conclusion
Transcription
This mystery can help answer a few questions in the field of cell biology such as protein input into chloroplasts. The main advantage of this technology is that protein input into chloroplasts can be studied under en vivo conditions. Demonstrating
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Here we describe a protocol to express proteins into protoplasts by using PEG-mediated transformation method. The method provides easy expression of proteins of interest, and efficient investigation of protein localization and the import process for various experimental conditions in vivo.