Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

7.4K Views

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08:33 min

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March 24th, 2019

DOI :

10.3791/59104-v

March 24th, 2019

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Seth A. Bennett¹^,², Samantha N. Cobos¹^,³, Marcella Meykler¹, Michel Fallah¹, Navin Rana¹, Karen Chen¹, Mariana P. Torrente¹^,⁴

¹Chemistry Department, Brooklyn College, ²Ph.D. Program in Biochemistry, Graduate Center of the City University of New York, ³Ph.D. Program in Chemistry, Graduate Center of the City University of New York, ⁴Ph.D. Programs in Chemistry, Biochemistry, and Biology, Graduate Center of the City University of New York

Summary

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Histone Post translational Modifications

Neurodegenerative Disease

Western Blot Apparatus

Overexpression Models

Liquid Culture

Optical Density OD600

Protein Overexpression

Cell Resuspension

Centrifugation

Induced Pluripotent Stem Cells

Chapters in this video

0:04

Title

1:00

Neurodegenerative Proteinopathy-associated Protein Overexpression in S. cerevisiae

3:02

Cell Lysis and Protein Transfer

5:28

Histone Post-translational Modification Detection

6:52

Results: Representative Effects of Yeast Protein Overexpression on Histone Expression and Modification

8:03

Conclusion

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