This set of standardizes protocols can be used to offer new insight into the superior ocular sulcus, or SOS, a recently discovered structure of the developing eye. These protocols will allow any experimenter around the world to clearly visualize t
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Here, we present a standardized series of protocols to observe the superior ocular sulcus, a recently-identified, evolutionarily-conserved structure in the vertebrate eye. Using zebrafish larvae, we demonstrate techniques necessary to identify factors that contribute to the formation and closure of the superior ocular sulcus.