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Live-imaging of the Drosophila Pupal Eye

DOI :

10.3791/52120-v

January 12th, 2015

January 12th, 2015

8,985 Views

1Biology Department, Wesleyan University

This protocol presents an efficient method for imaging the live Drosophila pupal eye neuroepithelium. This method compensates for tissue movement and uneven topology, enhances visualization of cell boundaries through the use of multiple GFP-tagged junction proteins, and uses an easily-assembled imaging rig.

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Live imaging

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