JoVE Logo
Faculty Resource Center

Sign In

Live-imaging of the Drosophila Pupal Eye

DOI :

10.3791/52120-v

January 12th, 2015

January 12th, 2015

8,996 Views

1Biology Department, Wesleyan University

This protocol presents an efficient method for imaging the live Drosophila pupal eye neuroepithelium. This method compensates for tissue movement and uneven topology, enhances visualization of cell boundaries through the use of multiple GFP-tagged junction proteins, and uses an easily-assembled imaging rig.

Tags

Live imaging

-- Views

Related Videos

article

Long-term Live Imaging of Drosophila Eye Disc

article

A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays

article

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

article

Live Imaging of Mouse Secondary Palate Fusion

article

Dissection and Staining of Drosophila Pupal Ovaries

article

Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

article

Live Imaging and Analysis of Muscle Contractions in Drosophila Embryo

article

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

article

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

article

Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved