Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry
8.9K Views
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11:54 min
•
July 13th, 2019
DOI :
July 13th, 2019
•Johannes Korth1,2, Helene Sertznig2, Siegfried Moyrer2, Adrian Atilla Nicolas Doevelaar2,3, Timm Henning Westhoff3, Nina Babel3, Oliver Witzke4, Andreas Kribben1, Ulf Dittmer2, Marek Widera2
1Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, 2Institute for Virology, University of Duisburg-Essen, University Hospital Essen, 3Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, 4Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr
Chapters in this video
0:00
Title
1:15
Collection of Blood and or Urine Samples and Isolation of BKPyV-DNA
2:11
Amplification and Sequencing of the Non-coding Control Region (NCCR)
3:37
Cloning of the Non-coding Control Region (NCCR) into the Dual Fluorescence Reporter
5:26
Transient Transfection of HEK293T Cells with the Dual Fluorescence Reporter Plasmid and Treatment with Potential Antiviral Agents
7:23
Fluorescence Microscopy and Flow Cytometry
9:22
Results and Data Interpretation
11:26
Conclusion
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