Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
8.8K Views
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09:15 min
•
July 18th, 2019
DOI :
July 18th, 2019
•Silvia Di Cocco1,2, Laura Belloni3, Abigail D.G. Nunn3, Debora Salerno3, Silvia Piconese2,4, Massimo Levrero2,3,5,6, Natalia Pediconi1,3
1Department of Molecular Medicine, Sapienza University, 2Department of Internal Medicine - DMISM, Sapienza University, 3Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, 4Pasteur Institute Italy-Fondazione Cenci Bolognetti, 5INSERM U1052-Cancer Research Center of Lyon, 6Department of Hepatology, Croix Rousse Hospital, Hospices Civils de Lyon
Chapters in this video
0:04
Title
0:32
Thawing, Amplification, and Cryopreservation of HepaRG Cells
2:20
Differentiation of HepaRG cells (Day 0 21) and Induction of Vesicular Steatosis (Day 21 26)
4:25
Evaluation of Lipid Overloading and Steatosis Induction: Oil Red O Staining
6:04
Evaluation of Lipid Overloading and Steatosis Induction: Bodipy Staining
7:02
Results: Induction and Quantification of Vesicular Steatosis in HepaRG Cells
8:34
Conclusion
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