Thawing, Amplification, and Cryopreservation of HepaRG Cells
2:20
Differentiation of HepaRG cells (Day 0 21) and Induction of Vesicular Steatosis (Day 21 26)
4:25
Evaluation of Lipid Overloading and Steatosis Induction: Oil Red O Staining
6:04
Evaluation of Lipid Overloading and Steatosis Induction: Bodipy Staining
7:02
Results: Induction and Quantification of Vesicular Steatosis in HepaRG Cells
8:34
Conclusion
필기록
This protocol describes how to induce liver vesicular steatosis in differentiated HepaRG cells and methods for detection and quantification of lipid accumulation. This in vitro human cell model represents a valuable alternative to in vivo mice mod
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In this study, we describe a detailed protocol for inducing liver vesicular steatosis in differentiated HepaRG cells with the fatty acid salt sodium oleate and employ methods for detection and quantification of lipid accumulation, including coherent anti-Stokes Raman scattering (CARS) microscopy, cytofluorimetric analysis, Oil red O staining, and qPCR.