JoVE Logo

Sign In

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

6.5K Views

06:52 min

July 22nd, 2020

DOI :

10.3791/61077-v

July 22nd, 2020


Explore More Videos

Driver Genes

Chapters in this video

0:04

Introduction

0:36

Cell Culture and Tumorsphere Formation

1:53

RNA Isolation

3:11

RNAseq Profiling and Bioinformatics Analysis

3:45

Driver Gene Selection

4:52

Results: Representative Tumorsphere Protein and Gene Expression Analyses

6:09

Conclusion

Related Videos

article

09:29

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

13.6K Views

article

06:19

Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer

9.9K Views

article

09:57

Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples

7.6K Views

article

07:18

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

6.9K Views

article

09:16

High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor

9.9K Views

article

07:01

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

9.9K Views

article

09:21

Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas

7.1K Views

article

09:24

Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells

4.3K Views

article

12:16

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

21.2K Views

article

06:38

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

5.9K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved